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| Contact |
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Truffer Frederic |
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| Title |
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Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells |
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| Author(s) |
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Truffer F., Buffi N., Merulla D., Beggah S., Lintel H., Renaud P., Roelof Meer J., Geiser M. |
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| References |
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Review of Scientific Instruments 85, 015120, January 2014 |
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| Url |
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http://scitation.aip.org/content/aip/journal/rsi/85/1/10.1063/1.4863333 |
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| Abstract |
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We present a compact portable biosensor to measure arsenic A (III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly re- producible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively. © 2014 AIP Publishing LLC. |
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