

Contact		Pfeifer Marc

Title		Construction of a Peptide Microarray for Auto-antibody

Author(s)		Vincent Cosandey, Fabien Debrot, Jérémy Kaeser, Roger Marti, Philippe Passeraub, Jannick Pétremand, Denis Prim, and Marc E. Pfeifer

References		Chimia, 2012, 66, No. 10

Url		http://dx.doi.org/10.2533/chimia.2012.803

Abstract		Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein-protein interactions (PPI), useful to study for instance antigen-antibody properties. Multivariate serology assays etecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).

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