| |
|
|
| |
|
|
| Contact |
|
Pfeifer Marc |
| |
|
|
| Title |
|
Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry |
| |
|
|
| Author(s) |
|
Denis Prim, Marc E. Pfeifer |
| |
|
|
| References |
|
Peptide Microarrays, Volume 1352 of the series Methods in Molecular Biology, pp 157-166 |
| |
|
|
| Url |
|
http://dx.doi.org/10.1007/978-1-4939-3037-1_12 |
| |
|
|
| Abstract |
|
Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays. |
| |
|
|
| |
|
|
| |
|
|
| |
|
|