| |
|
|
| |
|
|
| Contact |
|
Zinn Manfred |
| |
|
|
| Title |
|
Poly (4-hydroxybutyrate)(P4HB) production in recombinant Escherichia coli: P4HB synthesis is uncoupled with cell growth |
| |
|
|
| Author(s) |
|
Sylvaine Le Meur, Manfred Zinn, Thomas Egli, Linda Thöny-Meyer and Qun Ren |
| |
|
|
| References |
|
Microbial Cell Factories, 2013, 12:123 |
| |
|
|
| Url |
|
http://dx.doi.org/10.1186/1475-2859-12-123 |
| |
|
|
| Abstract |
|
Poly(4-hydroxybutyrate) (P4HB), belonging to the family of bacterial polyhydroxyalkanoates (PHAs), is a strong, flexible and absorbable material which has a large variety of medical applications like tissue engineering and drug delivery. For efficient production of P4HB recombinant Escherichia coli has been employed. It was previously found that the P4HB synthesis is co-related with the cell growth. In this study, we aimed to investigate the physiology of P4HB synthesis, and to reduce the total production cost by using cheap and widely available xylose as the growth substrate and sodium 4-hydroxybutyrate (Na-4HB) as the precursor for P4HB synthesis. |
| |
|
|
| |
|
|
| |
|
|
| |
|
|